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KMID : 0359319830230010009
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Korean Journal of Veterinary Research
1983 Volume.23 No. 1 p.9 ~ p.16
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Effects of Ethanol on Na - K - ATPase Activity of Cat Kidney
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Abstract
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The effects of ethanol on Na-K-ATPase activity were investigated with cat kidney homogenate.
The results were summarized as follows:
1. Na-K-ATPase activity was inhibited with dose-dependent manner by ethanol of higher concentration than 1%, and showed an estimated I_(50) (the inhibitor concentration to cause 50% inhibition) of 7.5%.
2. Hydrolysis of ATP was linear with the incubation time in the absence and presence of 8% ethanol, whereas it was different with preincubation time in the presence of 15% ethanol.
3. Inhibition of Na-K-ATPase activity by ethanol was not affected by increased enzyme concentration, and showed the reversibility of the inhibitory pattern.
4. Kinetic studies of cationic-substrate activation of Na-K-ATPase showed that ethanol had both properties of classical competitive inhibition for Mg＾(++) or 7＾+ and non-competitive inhibition for ATP or Na＾+.
5. Arrhenius plot yield two break point at 21℃ and 30℃ in the absence of ethanol, whereas showing only one break point at 18℃ in the presence of 8% ethanol.
These results suggested that ethanol inhibited Na-K-ATPase activity reversible through a disturbance of microenvironment of lipids associated with the enzyme.
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